Histone H3K4me1 antibody (pAb)

Catalog No: 39299 Format: 100 µl $510 Buy
Catalog No: 39300 Format: 10 µl $125 Buy
Catalog No: 39335 Format: 50 µl $295 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
17 kDa
Reactivity:
Wide Range Predicted

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

Application Notes

Applications Validated by Active Motif:
ChIP-Seq: 5 µl each ChIP
WB*:1:500-1:2000

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

For Histone H3K4me1, we also offer AbFlex® Histone H3K4me1 Recombinant Antibody (rAb) and a pAb. For details, see Catalog No. 91289, and Catalog No. 61781.

Immunogen

This antibody was raised against a peptide containing monomethyl-lysine 4 of Histone H3.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

 
Histone H3K4me1 antibody (pAb) tested by ChIP-Seq

Histone H3K4me1 antibody (pAb) tested by ChIP-Seq
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 15 µg of HeLa cell chromatin and 5 µl of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 14.1 million sequence tags were mapped to identify Histone H3K4me1 binding sites on chromosome 1. Histone H3K4me1 antibody Cat. No. 39299 is provided in a serum format. We also offer a Protein A purified IgG format of this antibody, Cat. No. 61781.

Histone H3K4me1 antibody (pAb) tested by Luminex bead-based specificity analysis.

Histone H3K4me1 antibody (pAb) tested by Luminex bead-based specificity analysis.
Various recombinant Histone proteins were conjugated to Luminex beads and incubated with varying dilutions of Histone H3K4me1 antibody. Bound antibody was detected with anti-rabbit IgG-PE and read in a MagPix Luminex Instrument.

Luminex® is a registered trademark of Luminex Corporation.

Histone H3K4me1 antibody (pAb) tested by Western blot.

Histone H3K4me1 antibody (pAb) tested by Western blot.
20 µg of HeLa cell nuclear extract was run on SDS-PAGE and probed with Histone H3K4me1 antibody at 1:2000.

Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.

Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K4me1 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting